Escherichia coli strain BL21 is one of the widely used bacterial hosts for high-level recombinant protein production and for other applications. Here, we present the complete genome sequence of a commercial version of the Escherichia coli BL21 strain. The four bcs-recombinant proteins were detected as the expected molecular weights in both induced cultures, but their overexpression was more pronounced in B21 (DE3) relative to DH5α cell lysates, in which no difference could be detected between induced and control culture profile. Given that BC is extracellularly expressed, it can be directly A one-way ANOVA with Dunnett's multiple comparison test was employed to evaluate differences in expression levels of recombinant protein between Evo21(DE3) and other expression hosts. P values BL21 (DE3) showed the highest level of expression in inclusion bodies followed by Rosetta-gami (DE3) and Shuffle T7. Changes of expression conditions were insufficient for soluble expression of reteplase in SHuffle T7 as a genetically engineered host for production of disulfide bonded proteins. .

difference between rosetta and bl21